绿花白千层叶油的GC-MS分析及其体外抑菌、抗炎活性研究

刘胜帅, 薛楠楠, 段银祥, 李婷, 段淑英, 刘沁攸, 张成桂, 刘衡

中国药学杂志 ›› 2019, Vol. 54 ›› Issue (16) : 1292-1298.

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中国药学杂志 ›› 2019, Vol. 54 ›› Issue (16) : 1292-1298. DOI: 10.11669/cpj.2019.16.003
论著

绿花白千层叶油的GC-MS分析及其体外抑菌、抗炎活性研究

  • 刘胜帅1, 薛楠楠1, 段银祥2, 李婷3, 段淑英2, 刘沁攸4, 张成桂1, 刘衡1*
作者信息 +

Analysis the Leaf Oil of Melaieuca viridiflora by GC-MS and Study on Its Antibacterial and Anti-inflammatory Activity

  • LIU Sheng-shuai1, XUE Nan-nan1, DUAN Yin-xiang2, LI Ting3, DUAN Shu-ying2,LIU Qin-you4, ZHANG Cheng-gui1, LIU Heng1*
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文章历史 +

摘要

目的 研究绿花白千层叶油成分及其抑菌、抗炎活性。方法 通过GC-MS对绿花白千层叶油进行分析,峰面积归一化法计算各成分相对百分含量;牛津杯法测定抑菌圈评价其对大肠埃希菌、金黄色葡萄球菌及白色念珠菌的抑菌性;建立脂多糖(LPS)诱导RAW264.7细胞炎症模型,采用酶联免疫吸附法检测细胞上清液中白细胞介素(IL)-1β及肿瘤坏死因子(TNF)-α的表达来考察其抗炎效果。结果 本实验鉴定出27个挥发性成分,占峰面积的95.23%,其中桉树醇含量最高,占40.38%;绿花白千层叶油对金黄色葡萄球菌、大肠埃希菌、白色念珠菌的抑菌效果分别属于极敏、高敏、中敏;绿花白千层叶油能显著抑制RAW264.7细胞分泌TNF-α、IL-1β(P<0.01),且具有剂量依赖性。结论 绿花白千层叶油成分复杂,且具有较强的体外抑菌、抗炎活性,其抗炎作用可能与阻止RAW264.7细胞向M1型极化,降低TNF-α、IL-1β的表达量有关。

Abstract

OBJECTIVE To investigate the composition of the leaf oil of Melaieuca viridiflora and its antibacterial and anti-inflammatory activities. METHODS The chemical components of the leaf oil of Melaieuca viridiflora were separated and identified by GC-MS, and the relative content of each component was determined by area nomalization. Through the Oxford cup method, the inhibition of Escherichia coli, Staphylococcus aureus and Candida albicans was evaluated by the inhibition zone. The lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was established. The anti-inflammatory effect of IL-1β and TNF-α was detected by enzyme-linked immunosorbent assay (ELISA). RESULTS In this experiment, 28 volatile components were identified, accounting for 95.23% of the total peak area, of which the highest content of eucalyptol was 40.38%. Staphylococcus aureus was extremely sensitive to the leaf oil of Melaieuca viridiflora, and Escherichia coli was highly sensitive to it. Candida albicans is moderately sensitive to the leaf oil of Melaieuca viridiflora. The leaf oil of Melaieuca viridiflora can significantly inhibit the secretion of TNF-α and IL-1β(P<0.01)by RAW264.7 cells and has a dose dependency. CONCLUSION The leaf oil of Melaieuca viridiflora has a complex composition and strong antibacterial and anti-inflammatory activity, and its anti-inflammatory effect may be related to preventing the differentiation of RAW264.7 cells into M1 type and reduce the expression of TNF-α, IL-1β.

关键词

绿花白千层 / 叶油 / 气相色谱-质谱 / 抑菌 / 抗炎

Key words

Melaieuca viridiflora / leaf oil / GC-MS / antibacteria / anti-inflammatory

引用本文

导出引用
刘胜帅, 薛楠楠, 段银祥, 李婷, 段淑英, 刘沁攸, 张成桂, 刘衡. 绿花白千层叶油的GC-MS分析及其体外抑菌、抗炎活性研究[J]. 中国药学杂志, 2019, 54(16): 1292-1298 https://doi.org/10.11669/cpj.2019.16.003
LIU Sheng-shuai, XUE Nan-nan, DUAN Yin-xiang, LI Ting, DUAN Shu-ying,LIU Qin-you, ZHANG Cheng-gui, LIU Heng. Analysis the Leaf Oil of Melaieuca viridiflora by GC-MS and Study on Its Antibacterial and Anti-inflammatory Activity[J]. Chinese Pharmaceutical Journal, 2019, 54(16): 1292-1298 https://doi.org/10.11669/cpj.2019.16.003
中图分类号: R917   

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基金

国家自然科学基金项目资助(81660605,81860742,81860765);药用特种昆虫开发国家地方联合工程研究中心创新能力建设项目资助(云发改高技395);云南省昆虫生物医药研发重点实验室专项经费资助(2015DG030);云南省2011协同创新中心项目资助(25)
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